Host Range, Cytology, and Transmission of an Alyce-clover Isolate of Blackeye Cowpea Mosaic Virus. G. S. Zhao, Graduate Research Assistant, Department of Agronomy and Plant Pathology, University of Florida, Gainesville 32611. D. D. Baltensperger, D. E. Purcifull, R. G. Christie, E. Hiebert, and J. R. Edwardson. Associate Professor, Professor, Plant Pathologist, and Professors, Departments of Agronomy and Plant Pathology, University of Florida, Gainesville 32611. Plant Dis. 75:251-253. Accepted for publication 4 September 1990. Copyright 1991 The American Phytopathological Society. DOI: 10.1094/PD-75-0251.

A blackeye cowpea mosaic virus (BlCMV) isolated from alyce-clover, designated as BlCMV-AC, was partially characterized. Cylindrical inclusions containing scrolls induced by BlCMV-AC were detected in infected plants by light, electron, and immunofluorescence microscopy. Isolate BlCMV-AC infected Alysicarpus vaginalis, Crotalaria spectabilis, Indigofera hirsuta, Lupinus albus, Macroptilium lathyroides, Nicotiana benthamiana, Phaseolus vulgaris ‘Black Turtle 2,’ Vigna unguiculata subsp. unguiculata ‘California Blackeye,’ Chenopodium amaranticolor, and C. quinoa. No seed transmission of BlCMV-AC was detected in alyce-clover, cowpea, or hairy indigo. Isolate BlCMV-AC was transmitted in a nonpersistent manner by Myzus persicae. Infection with BlCMV-AC, peanut stripe virus, or both significantly reduced plant height, dry weight of tops, and seed yield of both alyce-clover and hairy indigo but had no effect on nitrogen concentration of tops or roots. Genotypes of alyce-clover and hairy indigo reacted differently to infection by the two viruses, but all were altered in some growth parameters by viral infections.