VIEW ARTICLE

Molecular Plant Pathology

Infection of Theobroma cacao Using Cloned DNA of Cacao Swollen Shoot Virus and Particle Bombardment. Lynda S. Hagen, Station de Pathologie Végétale, Institut National de la Recherche Agronomique (INRA), B.P. 94, F-84143 Montfavet Cedex; Hervé Lot(2), Christian Godon(3), Mark Tepfer(4), and Mireille Jacquemond(5). (2)(5) Station de Pathologie Végétale, Institut National de la Recherche Agronomique (INRA), B.P. 94, F-84143 Montfavet Cedex; (3)(4)Laboratoire de Biologie Cellulaire, INRA, Route de St. Cyr, F-78026 Versailles Cedex, France. Phytopathology 84:1239-1243. Accepted for publication 25 April 1994. Copyright 1994 The American Phytopathological Society. DOI: 10.1094/Phyto-84-1239.

A full-length, monomeric genomic clone of cacao swollen shoot badnavirus was infectious after particle bombardment of cacao seeds from which the testae had been removed. The infected plants showed symptoms typical of the severe form of cacao swollen shoot disease. Progeny virus from plants infected by bombardment reacted in enzyme-linked immunosorbent assay and dot blot hybridization and had small bacilliform particle morphology as observed by immunosorbent electron microscopy. Furthermore, the complex of symptoms that characterize cacao swollen shoot disease can be provoked by a single etiologic agent rather than by a mixture of different viruses or strains. The infectious clone offers new possibilities for the study of swollen shoot disease.

Additional keywords: biolistics, inoculation, microprojectile bombardment.