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Semiselective Medium for Isolation of Cryphonectria cubensis. E. Conradie, Department of Plant Pathology, University of the Orange Free State, Bloemfontein 9300, South Africa; W. J. Swart(2), and M. J. Wingfield(3). (2)Department of Plant Pathology, University of the Orange Free State, Bloemfontein 9300, South Africa; (3)Department of Microbiology and Biochemistry, University of the Orange Free State, Bloemfontein 9300, South Africa. Phytopathology 82:1440-1442. Accepted for publication 29 July 1992. Copyright 1992 The American Phytopathological Society. DOI: 10.1094/Phyto-82-1440.

Twenty-seven compounds were tested in vitro for their effect on mycelial growth of Cryphonectria cubensis, the cause of canker of Eucalyptus spp. Eighteen were subsequently tested for their ability to suppress mycelial growth of 15 fungi commonly associated with C. cubensis in host tissue. Gallic acid and dicloran, respectively, were the most effective inhibitors of Trichoderma sp. and Mucor sp. A semiselective medium composed of 20 g of Difco BiTek agar, 10 g of d-glucose, 1 g of l-asparagine, 0.001 g a.i. of chlorothalonil, 0.0055 g a.i. of dicloran, 0.001 g a.i. of vinclozolin, 2 g of gallic acid, 0.1665 g of streptomycin sulphate, and 1 L of distilled water proved very effective for isolating C. cubensis from woody tissue and forest soil.

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