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Cytology and Histology

Development of Cylindrical Inclusions in Potyvirus-Infected Protoplasts. John F. Murphy, Department of Plant Pathology, University of Kentucky, Lexington 40546; Ulla Järlfors, and John G. Shaw. Department of Plant Pathology, University of Kentucky, Lexington 40546. Phytopathology 81:371-374. Accepted for publication 18 September 1990. Copyright 1991 The American Phytopathological Society. DOI: 10.1094/Phyto-81-371.

The development of cylindrical inclusions in tobacco mesophyll protoplasts infected with the potyvirus tobacco vein mottling virus (TVMV) was examined. Bundles and pinwheels were observed in ultrathin sections of protoplasts collected 15–30 h after inoculation. In sections subjected to immunogold labeling with anti-TVMV cylindrical inclusion protein serum and gold-conjugated Protein A, the cylindrical inclusion protein was detected as early as 10 h after inoculation.