VIEW ARTICLE

Techniques

The Use of Immuno-Gold Staining Techniques for Detection of a Bacterium Associated with Greening Diseased Citrus. Dvora Ariovich, Department of Microbiology, University of the Witwatersrand, Johannesburg, P.O. Wits, 2050, South Africa; Helen M. Garnett, Department of Microbiology, University of the Witwatersrand, Johannesburg, P.O. Wits, 2050, South Africa, Present address: Department of Biology, University of Wollongong, Northfields Avenue, Wollongong, NSW 2500, Australia. Phytopathology 79:382-384. Accepted for publication 22 July 1988. Copyright 1989 The American Phytopathological Society. DOI: 10.1094/Phyto-79-382.

An immunoelectron microscopic method using immuno-gold staining was developed for the detection of the putative greening organism in greening diseased citrus. This technique had the advantage of being rapid and highly specific and detected bacteria even when they occur in low numbers in infected tissue.