First Report of Colletotrichum acutatum Causing a Leaf Spot on Myrica cerifera in Florida. S. J. Mackenzie, University of Florida, Gulf Coast Research and Education Center, Wimauma 33598; L. M. Takahashi, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil; and J. C. Mertely, T. E. Seijo, and N. A. Peres, University of Florida, Gulf Coast Research and Education Center, Wimauma 33598. Plant Dis. 90:1263, 2006; published on-line as DOI: 10.1094/PD-90-1263B. Accepted for publication 30 June 2006.

Wax myrtle (Morella cerifera (synonym Myrica cerifera) (L.) Small) is a native tree used in Florida landscapes. In the summer of 2005 and spring of 2006, small necrotic spots were observed on young leaves in two commercial nurseries in central Florida. Lesions were dark brown-to-black and eventually coalesced to form large, irregular necrotic areas. Leaves with large lesions abscised prematurely, defoliating the entire plant. Conidia formed on acervuli were observed on the surface of the largest lesions and were tentatively identified as a Colletotrichum sp. Isolations from the edges of lesions were made on potato dextrose agar (PDA) after surface disinfestation of leaf pieces in 0.6% NaOCl for 30 sec. Red chromogenic colonies developed after 5 days of incubation at 24°C. Colonies produced hyaline, oblong conidia with pointed ends averaging 14 × 4 µm and were identified as Colletotrichum acutatum J.H. Simmonds (1). The sequence from internal transcribed spacer regions 1 and 2 and the 5.8s rRNA gene of the rDNA repeat for an isolate (GenBank Accession No. DQ839609) was 100% identical to sequence from the same region of 36 C. acutatum isolates in the NCBI database. These isolates came from at least 16 different hosts, including seven ornamental hosts. There were three isolates from blueberry among the matches (Accession Nos. AB219029, AJ301911, and AJ301905), and the rDNA sequence was also identical to the sequence obtained in our laboratory for a chromogenic C. acutatum isolate from blueberry. Three single-spore isolates were tested for pathogenicity on potted plants in the greenhouse. Two young shoots were spray inoculated with a suspension (1 × 10(^6) conidia/ml) of each isolate. Shoots were covered with a plastic bag for 24 h and maintained at 26.5°C. Two shoots were sprayed with sterile water as a control and similarly covered. All isolates produced brown spots on the youngest leaves 3 to 5 days after inoculation; no symptoms developed on control shoots. The fungus was reisolated from all inoculated shoots. To our knowledge, this is the first report of C. acutatum on wax myrtle in Florida. The disease has a potential to spread and become a significant problem for the cultivation of this species in ornamental nurseries in Florida.

Reference: (1) J. H. Simmonds. Qld. J. Agric. Anim. Sci. 22:437, 1965.