First Report of White Mold Caused by Sclerotinia sclerotiorum on Rosmarinus officinalis ‘Prostratus’ in Italy. A. Garibaldi, A. Minuto, and M. L. Gullino, Centre of Competence for the Innovation in the Agro-Environmental Sector (AGROINNOVA) Via Leonardo da Vinci 44, 10095 Grugliasco, Italy. Plant Dis. 89:1016, 2005; published on-line as DOI: 10.1094/PD-89-1016A. Accepted for publication 21 June 2005.

Rosmarinus officinalis L. ‘Prostratus’ is an evergreen shrub that is native to the Mediterranean Region in southern Europe and grown as a potted plant in Italy. This cultivar is widely used in gardens and landscapes. During the winter of 2002, extensive chlorosis was observed on 8-month-old potted plants of R. officinalis L. ‘Prostratus’ grown outdoors in commercial farms near Albenga in northern Italy. Initial symptoms included stem necrosis at the soil level and darkening of leaves. As stem necrosis progressed, infected plants wilted and died. Wilt, characterized by the presence of soft and watery tissues, occurred within a few days on young plants. The disease infected 15% of the plants. Necrotic tissues became covered with a whitish mycelium that produced dark sclerotia. The diseased stem tissue was surface sterilized for 1 min in 1% NaOCl and plated on potato dextrose agar (PDA) amended with 100 ppm of streptomycin sulfate. Sclerotinia sclerotiorum (1) was consistently recovered from infected stem pieces. Sclerotia observed on infected plants measured 0.30 to 3.33 × 1.00 to 4.23 mm (average 1.31 × 1.88 mm). Sclerotia produced on PDA measured 0.09 to 3.08 × 0.38 to 4.05 mm (average 1.94 × 2.43 mm). Pathogenicity of three isolates obtained from infected plants and used in mixture was confirmed by inoculating 60-day-old plants grown in 14-cm-diameter pots in a glasshouse. Inoculum (wheat kernels infested with mycelium and sclerotia) for each isolate was placed on the soil surface around the base of each plant. Pathogenicity tests included three inoculated plants grown in separate pots per isolate. Three noninoculated plants grown in three pots served as controls. The inoculation trial was conducted twice. All plants were kept at temperatures ranging between 8 and 34°C (average 18°C) and watered as needed. Plants were covered with plastic for 96 h after inoculation to increase the moisture level. All inoculated plants developed symptoms of leaf yellowing within 30 days, soon followed by the appearance of white mycelium and sclerotia, and eventual wilt. Control plants remained symptomless. S. sclerotiorum was reisolated from the stems of inoculated plants. To our knowledge, this is the first report of white mold of R. officinalis L. ‘Prostratus’ in Italy and in Europe. S. sclerotiorum has been previously reported on R. officinalis in India (2) and the United States (3). The economic importance of this disease for the crop in Italy can be considered low at the moment.

References: (1) N. F. Buchwald. Den. Kgl. Veterin.er-og Landbohojskoles Aarsskrift, 32:75, 1949. (2) L. Mohan. Indian Phytopathol. 47:443, 1994. (3) M. L. Putnam. Plant Pathol. 53:252, 2004.