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The American Phytopathological Society
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First Report of Tomato leaf curl New Delhi virus Infecting Tomato in
Bangladesh. M. N. Maruthi, A. R. Rekha, A. Cork, and J. Colvin, Natural
Resources Institute, University of Greenwich, Chatham Maritime, Kent ME4 4TB,
UK; and S. N. Alam and K. A. Kader, Bangladesh Agricultural Research Institute,
Joydebpur, Gazipur, Bangladesh. Plant Dis. 89:1011, 2005; published on-line as
DOI: 10.1094/PD-89-1011C. Accepted for publication 2 June 2005.
Tomato is an important cash crop for resource-poor farmers and accounts for
20% of the 2 million t of vegetables grown annually in Bangladesh. Tomato
cultivation is affected by Tomato leaf curl virus (ToLCV), which can
cause as much as 100% yield loss. Plants exhibiting typical ToLCV disease
symptoms of yellowing, severe leaf curling, and stunting were collected at
Jessore, Bangladesh during September 2003. The putative virus was transmitted
from tomato to tomato by the whitefly Bemisia tabaci. In two separate
experiments, 100% transmission was achieved by using 10 viruliferous B.
tabaci adults for each of the 20 test plants that was confirmed by comparing
the symptoms on test and virus source plants. Total DNAs were extracted from the
symptomatic leaves, and the putative viral genomes were amplified by polymerase
chain reaction by using the Deng A and B primers (1). Sequences generated from
these primers were used to design virus-specific primers that were used to
obtain complete viral sequences. Full-length DNA-A (2,740 nt; GenBank Accession
No. AJ875157) and DNA-B (2,688 nt; GenBank Accession No. AJ875158) sequences of
a bipartite Tomato leaf curl New Delhi virus from Jessore (ToLCNDV-[Jes])
were obtained, which were most similar to the corresponding sequences of
ToLCNDV-(Lucknow) (GenBank Accession No. Y16421) at 95.7% and Tomato leaf
curl Gujarat virus-(Varanasi) (GenBank Accession No. AY190291) at 90.6% nt
identities, respectively. DNA-A sequences had only 73.2% nt identity with the
previously reported monopartite Tomato leaf curl Bangladesh virus
(GenBank Accession No. AF188481) (2), confirming the occurrence of mono- and
bipartite begomoviruses in Bangladesh. The virus diversity poses a challenge
for ToLCVD management in Bangladesh.
References: (1) D. Deng et al. Ann. Appl. Biol. 125:327, 1994. (2) S. K.
Green et al. Plant Dis. 85:1286, 2001.
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