Oral: Contributions of Plant Viruses to Phytobiome Research
Plant-virus protein interaction and localization maps: Where's the game and who's on the team?
M. Goodin (1) (1) University of Kentucky, U.S.A.
Viruses pose constant threats to human health and welfare. My laboratory has focused primarily on Potato yellow dwarf virus (PYDV) and Sonchus yellow net virus (SYNV), two members of the genus Nucleorhabdovirus, defining characteristics of which are their replication in nuclei of infected cells and accumulation of mature virions in the perinuclear space after budding through the inner nuclear membrane. More recently, we have investigated the Dichorhavirus (“bipartite rhabdovirus”), coffee ringspot virus, which has emerged in the majority of the coffee producing areas in Brazil. In order to characterize these viruses, and with the help of collaborators, we developed several enabling technologies. The first of these, the pGD vectors, are described in a 2002 publication that is currently linked to 98 citations spanning a wide range of plant and microbial research. The subsequent second and third generation pSITE and pSITEII protein expression vectors are linked to 57 and 48 citations, respectively. Most of these vectors have been employed in experiments utilizing the model plant Nicotiana bethamiana, the first comprehensive review of which has been downloaded >1500 times from the MPMI website, where it remains the third most accessed publication on that site. Using examples from my own research and of others, the need for vastly expanded protein localization and interaction databases will be discussed.