Poster: Molecular & Cellular Plant-Microbe Interactions: Proteomics/Metabolomics/Genomics
Identification of defense-related genes associated with tomato Sw-7 line against Tomato spotted wilt virus in tomato through transcriptome analysis
C. PADMABHAN (1), Y. Zheng (2), R. Shekaste-band (3), K. Stewart (1), J. Scott (3), Z. Fei (2), K. Ling (1) (1) USDA-ARS, U.S. Vegetable Laboratory, U.S.A.; (2) Boyce Thompson Institute for Plant Research, U.S.A.; (3) University of Florida, IFAS, Gulf Coa
Tomato spotted wilt virus (TSWV) is a highly infectious tospovirus, and one of the most damaging plant viruses infecting tomatoes worldwide. Developing a tomato cultivar with TSWV-resistance would be the most effective approach for disease management. Comparative analysis of differential expression of genes in plant functional pathways between highly isogenic tomato breeding lines with striking difference in resistance and susceptibility to TSWV may lead to understanding of the mechanism of disease resistance. In the present study, RNA-seq was performed on samples from a near-isogenic breeding line containing Sw-7 and its recurrent TSWV-susceptible parent at 0, 4, 7, 14, 21 and 35 days post inoculation (dpi). Comparative transcriptome analysis revealed a large number of genes that are differentially expressed (DE) by TSWV infection over time, including pathogenesis related 1 (PR1) and 5 (PR5) (Osmotic-like protein), Pto-like, Serine/threonine kinase protein, N-methyltransferase-like protein, auxin, glycine/proline rich protein, MADS box transcription factors, histidine kinase, histone proteins, as well as the RNA silencing pathway genes, argonaute, DCL3 and PolIV and microRNA target genes. Genome-wide transcriptome analysis offers us an opportunity to understand the mechanism of disease resistance, which may lead us to an effective management of TSWV in tomato and other crops.