Poster: Diseases of Plants: Disease Detection & Diagnosis
Necrotic Streaking of Asiatic Lilies caused by Plantago asiatica mosaic virus in Chile
X. BESOAIN (1), A. Vidal Takasaki (2), R. Camps (2) (1) Pontificia Universidad Católica de Valparaíso, Chile; (2) Pontificia Universidad Católica de Valparaíso, Chile
During the spring of 2015 a survey was done in Quillota, in greenhouses with Asiatic hybrid lilies for cut flower. Symptomatic plants were found in four different cultivars. Symptoms were brown necrotic streaking associated with irregular leaf chlorosis, and severely infected plants also showed brown streaks in the stem and irregular necrosis in tepal tips. Only in cv. Litouwen it was observed that microbulbs replaced flower buds. Tissue extracts from symptomatic plants were obtained and RT-PCR of gene RdRp was performed employing specific primers for Potexvirus. The obtained sequences revealed 99% identity with the consensus sequences of PlAMV. After positive results for PlAMV, a pathogenicity test was performed on plants of two cultivars of Asiatic lilies. Two isolates of PlAMV were inoculated in three plants for each isolate, and other three plants were left as controls. Forty-five days after inoculation, plants from Asiatic cv. Indian Summerset showed a shorter stem, necrotic streaks in leaves and in the stems and chlorosis in irregular pattern. Litouwen plants showed symptoms of decreased growth, brown necrotic streaks in the leaves associated with chlorosis and microbulbs instead of flower buds. All symptomatic plants tested positive with specific primer for PlAMV and control plants tested negative. Koch’s postulates were fulfilled for PlAMV on Lilium plants and the disease was called Necrotic Streaking of Lily caused by PlAMV affecting Asiatic cultivars.