Poster: Biology & Disease Mgmt: Postharvest Pathology & Mycotoxins
Analysis of deoxynivalenol and deoxynivalenol-3-glucoside in Canadian spring wheat varieties inoculated with Fusarium graminearum
D. FERNANDO (1), C. Amarasinghe (1), S. Simsek (2) (1) University of Manitoba, Winnipeg, MB, Canada; (2) North Dakota State University, Fargo, ND, U.S.A.
Mycotoxins are a chemically diverse group of toxic secondary metabolites produced by fungi. In order to cope with these mycotoxins, plants have the potential to modify the chemical structures as part of their defense mechanism. The resulting metabolic products are neither routinely screened at grain elevators nor regulated by proper legislations. The objective of this study is to analyse the deoxynivalenol (DON) and deoxynivalenol-3-glucoside (D3G) content in Canadian spring wheat varieties grown in two locations, inoculated with different chemotypes of Fusarium graminearum. According to the analysis of variance, significant differences were observed among the varieties for fusarium head blight (FHB) disease index, fusarium damaged kernel percentage (%FDK), DON and D3G content. When considered the effect of chemotype, significant differences were observed for FHB disease index, %FDK and DON content. The D3G content and D3G/DON ratio were not significantly different between the chemotypes. Correlation analysis showed a strong positive correlation between DON and D3G. The highest D3G/DON ratio was observed in moderately resistant varieties. The susceptible varieties showed lower D3G/DON ratio compared to the moderately resistant varieties. The current study indicated that Canadian spring varieties produce D3G upon Fusarium infection and testing for D3G in food and feed safety assessments in Canada should be considered.