Poster: Biology & Disease Mgmt: Genetics of Resistance
Development of a molecular marker platform for the breeding of blast resistance varieties in Taiwan
W. CHEN (1), W. Shen (1), F. Chang (2), W. Chang (3), Z. Yu (1), J. Liao (1), M. Lai (4), C. Wu (2), C. Chung (1) (1) National Taiwan University, Taiwan; (2) Kaohsiung District Agricultural Research and Extension Station, Taiwan; (3) Taoyuan District Agri
Rice blast is the most important disease commonly occurring in the first cropping season in Taiwan. To facilitate marker-assisted backcrossing, this study aims to identify effective blast resistance (R) genes in Taiwan and develop R gene and background selection markers for the use of IRRI-bred blast resistant lines (IRBLs) as donors. The resistance spectra of known R genes were evaluated by inoculating 31 IRBLs with 18 M. oryzae isolates collected from six rice-growing areas in Taiwan in 2009-2013. The R alleles at Pi2/9, Pik, and Pita/Pita2 were resistant to more isolates. Genotyping-by-sequencing technology was used to analyze 13 IRBLs with broader-spectrum resistance and 20 Taiwan high-quality rice varieties. Based on the 131,942 SNPs between IRBLs and Taiwan varieties, a set of 11 polymorphic R gene markers and 96 Fluidigm markers evenly distributed on rice genome were developed. Two M. oryzae isolates 12YL-TT4-1 and 13TN-HB1-3 were used to determine which Taiwan varieties would be suitable recipients for the 13 R alleles. The result showed that Taikeng 4, Taikeng 9, Taikeng 10, Taikeng 14, Taikeng 15, Tainung 71, Taiyuan 3, Tainan 11, Kaohsiung 145, and Kaohsiung 147 do not carry the R alleles in IRBL9-W, IRBLz5-CA, IRBLz5-CA (R), IRBLz-Fu, IRBL1-CL, IRBL7-M, IRBLk-Ka, IRBLkp-K60, IRBLKm-Ts, IRBLkh-K3, IRBL20-IR24, IRBLta2-Pi, and IRBLta2-Re. The 13 IRBLs and newly developed markers can be used to improve blast resistance of the 10 high-quality rice varieties.