Poster: Biology & Disease Mgmt: Biological Control
Selection and characterization of probiotics for rye silage
C. JEON (1), H. Kim (1), J. Kim (1), Y. Kwak (2) (1) Division of Applied Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea, South Korea; (2) Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, R
Silage is feed of livestock. Forage crops are at risk from infection by mycotoxins producing fungi. Rye has considerate as a highly quality of forage. After heading stage, the biomass of rye increased up to 30%. However, lignification caused low fermentation efficiency and coefficient digestibility. This study was performed to develop probiotics which improve quality of silage in nutrient aspect. During the fermentation period of the rye silage, dominant microorganisms were selected. Those were confirmed antimicrobial activities against fungi and were tested acidification ability. The isolates confirmed ability of fibrinolysis through the four enzyme (cellulase, xylanase, esterase, chitinase). The final selected strains were added to the rye silage and then confirmed the effect by real-time PCR in antifungal activity. Total 180 isolates were isolated from the rye silages during various fermentation period. Among 180, 8 isolates showed antimicrobial activities against Fusarium moniliforme. two isolates among antimicrobial activities isolates showed strong acidification ability. Also a single isolate was selected that was effective all four enzymes. In real-time PCR results, the mycotoxin gene was detected significantly low in the rye silage, which added antimicrobial activity isolate. In case developed microorganism product was applied at other stock feed as well as rye silage, production of high quality forage will expand.