Poster: Molecular & Cellular Plant-Microbe Interactions: Proteomics/Metabolomics/Genomics
Comparison of Fusaruim graminearum transcription factors affecting Fusaruim graminearum virus 1 accumulation based on phenome analysis
J. Yu (1), Y. Lee (1), K. Kim (1) (1) Seoul National University, Korea
The Fusarium graminearum virus 1 (FgV1) causes hypovirulent phenotypic changes in Fusarium graminearum. It has been reported that the numerous F. graminearum genes were differentially expressed upon FgV1 infection including genes encoding regulatory factors. Using gene-deletion mutant library of 657 putative transcription factors (TFs) in F. graminearum, we transferred FgV1 by hyphal anastomosis to screen transcription factors that might be associated with viral replication or symptom induction. FgV1-infected TF mutants were divided into three groups according to the mycelial growth phenotype compare to the wild-type FgV1-infected strain (WT-VI). The FgV1-infected TF mutants in Group 1 exhibited slow or weak mycelial growth compare to that of WT-VI on CM media at 5-dpi. In contrast, Group 3 consists of virus-infected TF mutants showing faster mycelial growth and mild symptom compared to that of WT-VI. The hyphal growth of FgV1-infected TF mutants in Group 2 was not significantly different from that of WT-VI. These growth differences among the FgV1-infected TF mutant groups might be related with FgV1 RNA accumulations in infected host fungi. To confirm this, we tested FgV1 RNA accumulation of some of FgV1-infected TF mutants in each group. Taken together, our analysis will help us identifying regulator(s) of FgV1-triggered signaling and antiviral responses and thus expand our understanding on complex regulatory networks between FgV1 and F. graminearum interaction.