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2013 APS Annual Meeting Abstract

 

Oral Technical Session: Virus Characterization

68-O

Molecular biodiversity of tomato yellow leaf curl disease associated viruses in Saudi Arabia.
K. ALHUDAIB (1), A. Rezk (1), W. Alaraby (1)
(1) King Faisal University, Hofuf, Saudi Arabia

Tomato production in Saudi Arabia and anywhere is under constent threat of whitefly-transmitted begomoviruses that cause tomato yellow leaf curl disease (TYLCD) that has a large number of strains. Tomato samples with typical symptoms of TYLCD and other crops grown near tomato filed such as green bean and paper were collected from open fields and greenhouses from different locations in Saudi Arabia (Eastern Province, Qassim province, Hail and Jazan). PCR was used to identify the pathogen using degenerate primers of Geminiviruses (AVcore and ACcore). To determine exactly the virus strain, multiplex PCR was used with sets of specific primers for Tomato yellow leaf curl virus Almeria strain (TYLCV-Alm), Tomato yellow leaf curl virus Israel strain (TYLCV-IL), Tomato yellow leaf curl virus mild strain (TYLCV-mld) and Tomato yellow leaf curl virus Sardinia strain (TYLCSV). Most of tested tomato samples some of paper and green bean were positive with degenerate primers and negative with all other specific primers. PCR products were cloned and sequenced and the nucleotide sequences were submitted in gene bank under accession numbers KC428387, KC428388, KC432613, KC432614, KC432615, HM590558, KC479016 and KC479017. The phylogenic tree showed that all isolates tested were found to be related to Tomato leaf curl Iran, Yemen and Sudan viruses. The highest nucleotide identities (>95%) were obtained with members of the species from Iran and Yemen.

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