Development of a multivariate matrix to trace Clavibacter michiganensis subsp. michiganensis through tomato greenhouse operations
M. L. LEWIS IVEY (1), S. A. Miller (1), F. Baysal-Gurel (1), X. Xu (1), M. E. Bledsoe (2)
(1) Ohio State University, Wooster, OH, U.S.A.; (2) Village Farms International, Inc., Heathrow, FL, U.S.A.
Phytopathology 101:S101

Clavibacter michiganensis subsp. michiganensis (Cmm) is a seedborne pathogen that spreads rapidly through tomato greenhouse operations, causing significant losses. The genetic diversity of Cmm has been exploited in the past to discern its origin and distribution, but no formal traceability system has been developed. Using geographical information, propagation and production flow diagrams and varietal and seed source data, an industry-specific multivariate matrix that was superimposed with repPCR fingerprints of Cmm strains was designed. The multivariate matrix allows Cmm phenotypic and genotypic information to be recorded and transmitted at any specific point in the production system and the point of origin of each strain can be quickly identified. The efficacy of this system is currently being evaluated and four new Cmm clonal groups have been identified. Producer implementation of the multivariate matrix has the potential to improve production efficiency, improve phytosanitary practices by identifying possible control points in production, decrease disease management related costs and identify new and emerging strains of Cmm. This system will also allow for further advancement of our knowledge of the diversity and distribution of this pathogen throughout North America.