APS Abstract of Presentation

Allele mining for genes associated with partial resistance to Phytophthora sojae in soybean
H. WANG (3), S. Berry (3), S. K. St. Martin (2), L. Zhou (4), K. Krampis (4), D. Tucker (1), Y. Mao (4), I. Hoeschele (4), M. Maroof (1), B. Tyler (4), A. E. Dorrance (3)
(1) Crop and Soil Environmental Science, Virginia Tech, Blacksburg, VA; (2) The Ohio State University, Columbus, OH; (3) The Ohio State University, OARDC, Wooster, OH; (4) Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA
Phytopathology 98:S165

Phytophthora sojae causes soybean root and stem rot, resulting in an annual loss of 1–2 billion dollar in soybean production worldwide. Partial resistance confers a broad-spectrum durable resistance to P. sojae and serves as a more stable alternative than single gene mediated resistance. However, little is known about the molecular mechanisms behind it. In this study, microarray analysis was applied for global expression profiling of over 37500 transcripts in soybean during P. sojae infection, on a partial resistant soybean cultivar ‘Conrad’ and a susceptible cultivar ‘Sloan’. The contrast analysis revealed ~500 genes having significantly different expression pattern in ‘Conrad’ at 3 dai (day after inoculation) and ~1000 at 5 dai (P < 0.05), which implicates their potential roles in partial resistance. Four potential QTLs in MLGs F, H, J and L were identified in ‘Conrad’ from the 186 RILs of F(4:6) ‘Conrad’ × ‘Sloan’ population by single marker association (P < 0.01). These results infer that partial resistance in ‘Conrad’ is a complex trait that involves numerous genes whose expression is influenced by at least four loci.

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