APS Abstracts of Presentations

Effects of culture media on the growth of Phymatotrichopsis omnivora
Presenter: H.-K. Lee, The Samuel Roberts Noble Foundation, Inc., 2510 Sam Noble Parkway, Ardmore, OK 73401
Co-Author(s): S. Marek, Oklahoma State University, Department of Entomology & Plant Pathology, 127 Noble Research Center, Stillwater, OK 74078; M. Sledge, The Samuel Roberts Noble Foundation, Inc., 2510 Sam Noble Parkway, Ardmore, OK 73401
Phytopathology 95:1112

The growth rate of Phymatotrichopsis omnivora, the fungus causing “Phymatotrichum” or “cotton root rot”, was examined on eight culture media: LB agar containing malt extract (LB+M), corn meal agar (C), corn meal peptone yeast agar (CPY), water agar Fisher brand (FW), water agar Sigma brand (SW), potato dextrose agar (PDA), yeast peptone malt extract agar (YPM) and yeast peptone sucrose agar (YPS). These media were prepared in a 100 × 15 mm square style with 13 mm grid petri dish. Two P. omnivora strains from Texas cotton (TXCO3) and Oklahoma alfalfa (OKAlf) were tested. Plugs from water agar cultures of the fungus were used to inoculate the center of square grid petri dishes containing the test media. After incubating at 28°C, linear growth was recorded weekly for 30 days. Growth rates differed amongst the test media and between the strains. Growth was most rapid on PDA and the OKAlf strain grew more rapidly than TXCO3.

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