Phytopathology
Classic: Concerning a Contagium Vivum Fluidium as a Cause
of the Spot-Disease
of Tobacco Leaves
by Martinus W. Beijerinck
Historical view of
TMV-related research:
The Discovery of the Causal Agent of the
Tobacco Mosaic
Disease by Milton Zaitlin
Lessons in plant
pathology:
Tobacco
mosaic
by Karen-Beth G. Scholthof
APSnet
Education Center
Reviewed feature article
Scholthof, K.-B.G.
2001. 1898 - The beginning of Virology...time marches on. The Plant
Health Instructor. DOI: 10.1094/PHI-I-2001-0129-01.
1898 -The beginning of Virology...time marches on.
Prepared
by
Karen-Beth G. Scholthof
Texas A&M University
In
1898, Martinus W. Beijerinck, a Professor of Microbiology at the Technical
University at Delft, the Netherlands, put forth his concepts that viruses
were small and infectious. He defined the infectious agent as a "contagium
vivum fluidum", after he discovered that the virus readily passed
through a porcelain filter, suggesting that it was smaller than bacteria.
He also observed that the 'agent' could diffuse through agar that retained
bacteria, and furthermore, that the virus could not be cultured except in
living, growing plants. The virus was Tobacco mosaic virus (TMV).
This report, suggesting that 'microbes' need not be cellular, was to
forever change the definition of pathogens. In 1898, two Germans,
Loeffler and Frosch, former students of Koch, also used a porcelain filter
for the isolation of the causal agent of foot and mouth disease of cattle,
but they suggested that it was a very small microbe. Beijerinck was almost
alone in his forward thinking conclusions that he had a filterable,
invisible infectious agent that differed from small microbes. In
fact, it took 50 years to prove that TMV 
was an infectious
nucleoprotein. In that time, TMV was the first virus to be purified
in pure crystal form, the first pathogen to be passed through filter
candles, and the first virus to be identified as composed of an infectious
nucleic acid. Since then many viruses have been discovered in plants,
animal, fungi, and bacteria. TMV would continue to play a leading role in
the development of fundamental concepts in virology. In the 1960s and
1970s, TMV was a key component in the shift to molecular work in viruses,
particularly with regard to the understanding of genetic information and
the biological role of virus encoded proteins.
The history of TMV roughly parallels the early history of the American Phytopathological Society (APS) that was founded in 1908 and celebrates its 90th anniversary this year. At the 50th anniversary meeting of APS, W. M. Stanley (APS member, Nobel prize 1946 for crystallization of TMV) commented that the papers on TMV "demonstrate in no uncertain terms the great debt that the field of general virology owes to plant virus research" (REFERENCE). At this same meeting, J. G. Horsfall stated: "The tobacco mosaic virus is leading us and will surely lead us further into a knowledge of what life itself really is."
As part of the celebration of a century of virology, a TMV symposium (REFERENCE) was held at the Royal Society of Edinburgh in August 1998. Links to some of the original research on TMV, discussion regarding its significance to virology, and the significance of TMV as a plant pathogen are provided below.
Selected
References
Beijerinck,
M.W. 1898. Concerning a contagium vivum fluidium as a cause of the
spot-disease of tobacco leaves. Reprint from: Phytopathology Classics,
Number 7. 1942. James Johnson, translator, APS Press. CLICK
HERE TO DOWNLOAD THE FULL TEXT OF THIS WORK.
Creager, A.N., K.-B.G. Scholthof, V. Citovsky, H.B. Scholthof. 1999. Tobacco mosaic virus: Pioneering research for a century. Plant Cell. 11:301-308. CLICK HERE TO GO TO THE FULL TEXT OF THIS ARTICLE.
Holton, C.S., G. W. Fischer, R. W. Fulton, H. Hart, and S. E. A. McCallan (eds.). 1959. Plant Pathology: Problems and Progress, 1908-1958. University of Wisconsin Press.
Scholthof, K.-B.G. 2000. Tobacco mosaic. The Plant Health Instructor. DOI: 10.1094/PHI-I-2000-1010-01. CLICK HERE TO GO TO THIS WORK.
Scholthof, K.-B.G., J.G. Shaw, M. Zaitlan (eds.). 1999. Tobacco mosaic virus: One hundred years of contributions to virology. APS Press. CLICK HERE FOR MORE INFORMATION.
Zaitlin, M. 1998. The discovery of the causal agent of the tobacco mosaic virus disease. In: "Discoveries in Plant Pathology," S.D. Kung and S.F. Yang (eds.). World Publishing Co., Ltd. Hong Kong. Pp. 105-110. CLICK HERE FOR THE FULL TEXT OF THIS WORK.
© Copyright 2001 by The American Phytopathological Society. This article was first published July 1, 1998. It was peer reviewed, revised, and published as a feature article for The Plant Health Instructor January 29, 2001.
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