Figure 6. Experimental inoculation on the stem of a potted Datura stramonium (Jimson weed) plant with A. tumefaciens (electron micrograph). After 2-3 weeks, a crown gall tumor is generated. The integration of the T-DNA originating from the Ti plasmid harbored in A. tumefaciens is visualized by in situ T-DNA-DNA hybridization of the crown gall chromosome within gall tissue. The T-DNA was labeled with tritium and the integrated T-DNA hybridization is seen as a dark band (white arrow) as detected by x-ray emulsion film layered on the chromosomes from cells in crown gall. (Courtesy C. Kado)

Figure 6. Experimental inoculation on the stem of a potted Datura stramonium (Jimson weed) plant with A. tumefaciens (electron micrograph). After 2-3 weeks, a crown gall tumor is generated. The integration of the T-DNA originating from the Ti plasmid harbored in A. tumefaciens is visualized by in situ T-DNA-DNA hybridization of the crown gall chromosome within gall tissue. The T-DNA was labeled with tritium and the integrated T-DNA hybridization is seen as a dark band (white arrow) as detected by x-ray emulsion film layered on the chromosomes from cells in crown gall. (Courtesy C. Kado)

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