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DNA the Easy Way
(and “Gram Stain” Without the Mess)
Supplemental Information
and References
Supplementary information:
Sources of bacterial cultures:
Bacterial cultures can be obtained from various sources, including the
American Type Culture Collection, Carolina Biological Supply, some K-12
Mentors listed in this site, and individual researchers.
American Type Culture Collection
10801 University Boulevard
Manassas, VA 20110-2209
Address for products and services:
American Type Culture Collection
P. O. Box 1549
Manassas, VA 20108-1549
To order cultures or check order status:
Phone: 1-800-638-6597 (USA and Canada)
e-mail: sales@atcc.org
status@atcc.org
http://www.atcc.org
Carolina Biological Supply Company
2700 York Road
Burlington, NC 27215-3398
USA and Canadian customers:
Call: 1-800-334-5551
FAX: 1-800-222-7112
e-mail: carolina@carolina.com
http://www.carolina.com
Books:
Carroll, J. E. 1994. Learning Biology
with Plant Pathology, National Association of Biology Teachers (NABT),
Reston, VA. (This is a good general reference for laboratory safety and
procedure, culture media preparation, and how to grow bacterial
cultures.)
Needham, C., Hoagland, M., McPherson, K.,
and Dodson, B. 2000. Intimate Strangers: Unseen Life on Earth,
ASM Press, Washington, D.C. (Companion book to the PBS four part science
documentary of the same name. This illustrated book was written for a
general audience and provides an excellent introduction to the world of
microbes.)
Websites:
1. The Structure of DNA
http://www.accessexcellence.org/AB/GG/structure.html
2. The Basics of Agricultural Biotechnology
http://www.agriculture.purdue.edu/teachers/
3. The American Society of Microbiology (ASM) on its website at http://www.microbeworld.org/ lists a number of resources for teachers: http://www.microbeworld.org/resources/educate.aspx including a series of microbiological experiments http://www.microbeworld.org/resources/experiment.aspx. In addition, a listing of many additional items, some freely available and some by-subscription, may be found at http://www.microbelibrary.org/
4) The Microbe Zoo
http://commtechlab.msu.edu/sites/dlc-me/zoo/
Excellent website on microorganisms written by scientists at Michigan
State University.
Click on an area of the Microbe Zoo map, e.g. Dirtland, Animal
Pavillion, Water World, etc., to learn more about microorganisms that
live in those habitats.
5) Bugs in the News
http://people.ku.edu/~jbrown/bugs.html
Collection of special topics about microorganisms. You may have heard
about a particular microbe, e.g. E. coli in hamburger, Helicobacter
pylori and gastrointestinal reflux disease (GIRD), in the news and
would like to know more about it. This is the place to go to find out
more! Interesting and informative.
References:
Carroll, J. E. 1994. Learning Biology with Plant Pathology, National
Association of Biology Teachers (NABT), Reston, VA.
Ryu, E. 1938. On the Gram-differentiation of bacteria by the simplest method.
J. Jpn. Soc. Vet. Sci. 17:31.
Schumann, G. L. 1991. Plant Diseases: Their Biology and Social Impact.
APS Press, St. Paul, MN. Chapter 5. Genes and Genetic engineering, pp. 95-121.
Suslow, T. V., Schroth, M. N., and Isaka, M. 1982. Application of a rapid
method for gram differentiation of plant pathogenic and saprophytic bacteria
without staining. Phytopathology 72:917-918.
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