Bacterial Signaling: Identification of N-Acyl-Homoserine Lactone-Producing Bacteria.
MEDIA RECIPES AND BACTERIAL STRAINS
Phosphate Buffered Saline (PBS)
Per 1,000-ml:
- 1.6 g of NaCl
- 0.2 g of KH2PO4
- 2.9 g of Na2HPO4-12H2O
- 800 ml of deionized H2O
- Adjust pH to 7.4
- Adjust final volume to 1,000 ml
Luria Broth and Agar Plates
For 1,000-ml:
- 10 g of Tryptone
- 5 g of Yeast Extract
- 5 g of NaCl
- deionized H2O to 1,000 ml
- For agar plates add 15 g of agar
Glass Spreaders
Take a glass rod approximately 6 inches long. Heat an area approximately 2 inches from one end over a flame until it can be bent to a 90-degree angle. Let cool.
Bacterial Strains
The following Pseudomonas aureofaciens strains are available on request.
Strain 30-84:
Wild-type isolate that produces the orange phenazine antibiotics.
Strain 30-84I:
A derivative of strain 30-84 that does not produce AHL signal. It will produce phenazines if certain exogenous AHL signals are provided.
Strain 30-84Ice:
A derivative of strain 30-84 that does not produce phenazines, but does produce AHL signal.
Strain 30-84Gac:
A derivative of strain 30-84 that does not produce any AHL signal.
These strains can be obtained by
writing
Dr. Leland S. Pierson III
Department of Plant Pathology
Forbes 204
University of Arizona
Tucson, AZ 85721-0036
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