Nutrient Broth Yeast extract Medium (NBY) (12)
Per liter add: nutrient broth 8 g yeast extract 2 g K2HPO4 (anhydrous) 2 g KH2PO4 0.5 g Distilled H2O 974 ml For NBY agar add: Agar 15 g Add after autoclaving 20% glucose 25 ml 1 M MgSO4 1 ml
Per liter add:
nutrient broth
8 g
yeast extract
2 g
K2HPO4 (anhydrous)
KH2PO4
0.5 g
Distilled H2O
974 ml
For NBY agar add:
Agar
15 g
Add after autoclaving
20% glucose
25 ml
1 M MgSO4
1 ml
SOC Medium (10)
Per liter add: Bacto-tryptone 20 g Bacto-yeast extract 5 g NaCl 0.5 g 250 mM KCl 10 ml Distilled H2O 970 ml Adjust the pH to 7.0 with 5 N NaOH ~0.2 ml Add after autoclaving 1 M MgCl2 5 ml 1 M glucose 25 ml
Bacto-tryptone
20 g
Bacto-yeast extract
5 g
NaCl
250 mM KCl
10 ml
970 ml
Adjust the pH to 7.0 with 5 N NaOH
~0.2 ml
1 M MgCl2
5 ml
1 M glucose