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Electroporation and marker exchange of Pseudomonas syringae pv. syringae.
Laboratory Exercise I: Introduction of broad host range plasmids into P. syringae pv. syringae strain B301D.
Laboratory Exercise II: Introduction of a broad host range plasmid into various Pseudomonas syringae pv. syringae strains.
Laboratory Exercise III: Introduction of mutated genes into Pseudomonas syringae pv. syringae strain B301D, selection for marker exchange, and screening strains for phenotypic changes.
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Electroporation and marker exchange of Pseudomonas syringae pv. syringae.
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Section III, Note 7:
Section III, Note 7:
A good protocol for isolation of bacterial genomic DNA can be found in Ausubel et al.
(1)
and a protocol for Southern hybridization can be found in Sambrook et al.
(10).