K-12
Introductory
Advanced
Illustrated Glossary
Instructor Communication
Translations
APSnet Feature Articles
Lessons and Laboratories
Mentors
News and Views
Programs and Workshops
Resource Guide
DeBary Children's Science Book Award
Introduction to the Pathogen Groups
Plant Disease Lessons
Laboratory Exercises
Topics in Plant Pathology
Case Studies
Hungry Planet: Stories of Plant Diseases
APSnet Feature Articles
Resource Guide
Plant Disease Mngmt Simulations
APSnet Feature Articles
Laboratory Exercises
Resource Guide
Topics in Plant Pathology
Plant Disease Mngmnt. Simulations
A-D
E-H
I-M
N-R
S-V
W-Z
Restricted Area for Instructors
Teaching Articles
Teaching Notes
Home
|
Log In
QUICK LINKS
Join APS
Renew Membership
Buy a Book
APS Journals
Event Calendar
Member Directory
Electroporation and marker exchange of Pseudomonas syringae pv. syringae.
Laboratory Exercise I: Introduction of broad host range plasmids into P. syringae pv. syringae strain B301D.
Laboratory Exercise II: Introduction of a broad host range plasmid into various Pseudomonas syringae pv. syringae strains.
Laboratory Exercise III: Introduction of mutated genes into Pseudomonas syringae pv. syringae strain B301D, selection for marker exchange, and screening strains for phenotypic changes.
APSnet
>
Education
>
Advanced
>
Laboratory Exercises
>
Electroporation and marker exchange of Pseudomonas syringae pv. syringae.
>
Section II, Note 7:
Section II, Note 7:
All bacterial and fungal strains listed in this exercise, as well as plasmid constructs and vectors may be obtained from Brenda K. Scholz-Schroeder, POB 646430, Department of Plant Pathology, Washington State University, Pullman, WA 99163.