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Electroporation and marker exchange of Pseudomonas syringae pv. syringae.
Laboratory Exercise I: Introduction of broad host range plasmids into P. syringae pv. syringae strain B301D.
Laboratory Exercise II: Introduction of a broad host range plasmid into various Pseudomonas syringae pv. syringae strains.
Laboratory Exercise III: Introduction of mutated genes into Pseudomonas syringae pv. syringae strain B301D, selection for marker exchange, and screening strains for phenotypic changes.
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Electroporation and marker exchange of Pseudomonas syringae pv. syringae.
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Section II, Note 3:
Section II, Note 3:
Electroporation efficiency is cell density dependent up to 1 x 10
10
CFU/ml. As long as the cell concentrations do not go below 1 x 10
10
CFU/ml, the variations in electroporation efficiencies are usually dependent on other factors such as bacterial strain, resistance, voltage, and DNA concentration
(3)
.