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Share APSnet > Careers > Job Center Molecular diagnostics of Phytophthora Job Title Job Category FieldOfStudy Job Details Job Requirements Job Materials Pay Scale Closing Date Closing Date Detail Company Organization Type City Location Region State/Province Country/Region Organization Description Web Page Contact Address 1 Contact Address 2 Contact City Contact Email Contact Fax Contact First Name Contact Last Name Contact Phone Contact State Contact Zip Contact Country Contact Organization Location Preference End Date Approval Status Attachments Content Type: APSJobOpenings Created at by Last modified at by Use this page to add attachments to an item. Name     Close Job Number 566 Job Title Molecular diagnostics of Phytophthora Job Category Postdoc Field Of Study Any Job Details There will be a post doctoral position working on molecular diagnostics and mitochondrial genome evolution opening up in Salinas, California in the near future. The project is to expand on the Phytophthora diagnostic marker system based on gene order differences that were recently developed. Basically mitochondrial gene order differences were identified in sequenced mt genomes for 19 Phytophthora spp. compared to 14 Pythium and multiple plant species to identify highly conserved regions for designing the markers. Two different loci were identified for further work and both have a single primer pair with multiplexed TaqMan probes for simultaneous detection at a genus and species specific level. Due to a higher level of interspecific sequence variation in one locus it has been the focus of the development of species specific probes while the second locus has been used primarily just for a second genus specific detection capability. There is a large sequence database for nearly all described Phytophthora spp. to support this project. The post doc's primary project would be to: 1) validate additional species specific TaqMan probes, especially for species of regulatory concern to APHIS - 14 probes have already been validated and a list of other possible species-specific probes has been generated. 2) modify the locus for use with isothermal amplification techniques in an effort to simplify the use of these diagnostic markers. We have ongoing projects looking at oomycete mitochondrial genome evolution and phylogenetics, development of diagnostic markers for Pythium using the same approach as outlined above for Phytophthora, as well as development of mitochondrial haplotype markers for Phytophthora that they could also participate in if they are interested. The USDA-ARS does have some citizenship requirements for hiring (for example, citizens of China or India will not be able to be hired but citizens from a number of other countries can be; please see http://www.afm.ars.usda.gov/hrd/jobs/VISA/countries.htm). Job Requirements Experience working with molecular techniques essential, prior mycological experience and working with TaqMan real time PCR desirable. Job Materials Please send an e-mail indicating interest and forward CV and reprints Pay Scale Closing Date Closing Date Detail Open until the position is filled Company USDA-ARS Organization Type Federal Government City Salinas Location Region West (AK, CA, CO, HI, ID, MT, NV, OR, UT, WA, WY) State/Province California Country/Region USA Organization Description Federal Research Station Web Page http://www.ars.usda.gov/main/site_main.htm?modecode=53-05-00-00 Contact Organization USDA-ARS Contact First Name Frank Contact Last Name Martin Contact Address 1 1636 East Alisal St. Contact Address 2 Contact City Salinas Contact State CA Contact Zip 93905 Contact Country USA Contact Email frank.martin@ars.usda.gov Contact Fax (831) 755-2814 Contact Phone (831) 755-2873 Attachment Close

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