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Plant Tissue Culture, Development, and Biotechnology

Robert N. Trigiano and Dennis J. Gray

"The Editors are to be complimented on bringing together the views of so many of the World's current experts in this field... The Editors tried to assign each Chapter to one or a group of experts in the field. This has ensured broad/in-depth coverage of the various topics selected...very comprehensive with much to offer the students, teachers and researchers. It is a valuable contribution to possess and it will remain of value for a very long time. It would be advisable for all Libraries to have copies on their shelves."
-- South African Journal of Botany

Item No: 83262

$119.95 Price $107.96 Member Price

Addressing several specialized areas generically termed biotechnology, Plant Tissue Culture, Development and Biotechnology uses a broad range of species that are studied via laboratory exercises. Based on the editors’ previous textbooks Plant Development and Biotechnology and Plant Tissue Culture Concepts and Laboratory Exercises – Second Edition, the book provides detailed perspectives and hands-on training in this rather diverse field. It concentrates on plant development and its application in biotechnology, while maintaining as broad of a base as possible. Supporting laboratory exercises are provided.

Integrates traditional plant sciences with recent advances plant tissue culture, development and biotechnology
Includes critical thinking questions, concept boxes introducing important ideas in the chapters, and procedure boxes giving precise instruction for experiments
New chapters, concepts, and laboratory exercises provide information on germplasm preservation, plant growth regulators, proper microscope use with digital photography, embryo rescue, micropropagation of roses, haploid cultures, and transformation of meristems
Laboratory exercises provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed to perform them
Special topics highlight copyrights, patents, legalities, and trade secrets in biotechnology

Introduction

D. J. Gray and R. N. Trigiano

Supporting Methodologies and General Concepts

Getting Started with Tissue Culture—Media Preparation, Sterile Technique, and Laboratory Equipment

Caula A. Beyl

Nutrition of Cell and Organ Cultures

J. D. Caponetti and R. N. Trigiano

PGRs and Their Use in Micropropagation Caula A. Beyl

Elements of In Vitro Research

Michael E. Compton

Proper Use of Microscopes

David T. Webb

Plant Histological Techniques

R. N. Trigiano, D. J. Gray, K. R. Malueg, K. A. Pickens, Z.-M. Cheng, and E. T. Graham

A Brief Introduction to Plant Anatomy and Morphology

R. N. Trigiano, Jennifer A. Franklin, and D. J. Gray

Seed Development and Germination

Feng Chen, Ruth C. Martin, Songquan Song, and Hiroyuki Nonogaki

Molecular Tools for Studying Plant Genetic Diversity

Timothy A. Rinehart, Xinwang Wang, R. N. Trigiano, Naomi R. Rowland, and Rene E. DeVries

Molecular Approaches to the Study of Plant Development

Albrecht G. von Arnim and Byung-Hoon Kim

Propagation and Development Concepts

Propagation by Shoot Culture

Michael Kane

Micropropagation of Syngonium by Shoot Culture

Michael Kane

Micropropagation and In Vitro Flowering of Rose

Michael Kane, Timothy Johnson, and Philip Kauth

Micropropagation of Potato by Node Culture and Microtuber Production

Michael Kane

Commercial Laboratory Production

G. R. L. Suttle

Detection and Elimination of Microbial Endophytes and Prevention of Contamination in Plant Tissue Culture

Alan C. Cassells

Culture Indexing for Bacterial and Fungal Contaminants

Michael Kane, Philip Kauth, and Timothy Johnson

Propagation from Nonmeristematic Tissues—Organogenesis

Robert L. Geneve

Developing a Molecular Understanding of In Vitro and In Planta Shoot Organogenesis

Ling Meng, Shibo Zhang, and Peggy G. Lemaux

Direct Shoot Organogenesis from Leaf Explants of Chrysanthemum and African Violets

R. N. Trigiano, L. M. Vito, M. T. Windham, Sarah Boggess, and Denita Hadziabdic

Propagation from Nonmeristematic Tissues—Nonzygotic Embryogenesis

D. J. Gray

Developmental and Molecular Aspects of Nonzygotic (Somatic) Embryogenesis

Xiyan Yang and Xianlong Zhang

Embryogenic Callus and Suspension Cultures from Leaves of Orchardgrass

D. J. Gray, R. N. Trigiano, and Bob V. Conger

Direct Nonzygotic Embryogenesis from Leaves and Flower Receptacles of Cineraria

R. N. Trigiano, M. C. Scott, and K. R. Malueg

Crop Improvement Techniques

Protoplasts—An Increasingly Valuable Tool in Plant Research

Jude W. Grosser and Ahmad A. Omar

Demonstration of Principles of Protoplast Isolation Using Chrysanthemum and Orchardgrass Leaves

R. N. Trigiano

Isolation, Culture, and Fusion of Tobacco and Potato Protoplasts

Richard E. Veilleux and Michael E. Compton

Haploid Cultures

Denita Hadziabdic, Phillip A. Wadl, and Sandra M. Reed

Production of Haploid Tobacco and Potato Plants Using Anther Culture

Phillip A. Wadl, Sandra M. Reed, and Denita Hadziabdic

Embryo Rescue

Tom Eeckhaut, Katrijn Van Laere, and Johan Van Huylenbroeck

Promoters and Gene Expression Regulation

Zhijian T. Li and D. J. Gray

Genetic Engineering Technologies

Zhijian T. Li, Sadanand A. Dhekney, and D. J. Gray

Transformation of Plant Meristems

Jean H. Gould

Genetic Transformation of Chysanthemum and Tobacco Using Agrobacterium tumefaciens

Margaret M. Young, Linas Padegimas, Nancy A. Reichert, and R. N. Trigiano

Genetic Transformation of Tobacco and Production of Transgenic Plants

Sadanand A. Dhekney, Zhijian T. Li, and D. J. Gray

Genetically Modified Controversies

H. A. Richards, L. C. Hudson, M. D. Halfhill, and C. N. Stewart, Jr.

Cryopreservation of Plant Cells, Tissues, and Organs

Barbara M. Reed, M. N. Normah, and Svetlana V. Kushnarenko

Cryopreservation of In Vitro Grown Shoot Tips

Svetlana V. Kushnarenko, Barbara M. Reed, and M. N. Normah

Cryopreservation of Orthodox and Recalcitrant Seed

M. N. Normah, W. K. Choo, Svetlana V. Kushnarenko, and Barbara M. Reed

Plant Biotechnology for the Production of Natural Products

Ara Kirakosyan, E. Mitchell Seymour, and Peter Kaufman

Pigment Production in Ajuga Cell Cultures

Mary Ann Lila and Randy B. Rogers

Variation in Tissue Culture

Margaret A. Norton and Robert M. Skirvin

The Business of Biotechnology

Biotechnology Entrepreneurship in the 21st Century

David W. Altman

Intellectual Property Protection for Plants

Chris Eisenschenk

Index

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